An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies

An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies

The effectiveness of therapeutic monoclonal antibodies (mAbs) is governed not only by their bioactivity, but also by their biophysical properties. Assays for rapidly evaluating the biophysical properties of mAbs are valuable for identifying those most likely to exhibit superior properties such as high solubility, low viscosity and slow serum clearance. Analytical hydrophobic interaction chromat...

Using Hydrophilic Interaction Chromatography for Heightened Product Characterization to Overcome Challenges with Hydrophobic Monoclonal Antibodies and Antibody Drug Conjugates

■ ■ Reduced temperature dependence for decreasd on-column degradation IN T RO DU C T IO N While hydrophilic interaction chromatography (HILIC) has been widely used for separating small polar compounds, its application to large biomolecules, other than released glycans, has been surprisingly limited. In large part, this can be attributed to a paucity of suitable HILIC column technology and robus...

Production and Partial Characterization of Monoclonal Antibodies to Leishmania

Background: Monoclonal antibody technology allows identification of amastigote-specific antigen in human tissue biopsies and search for a suitable vaccine candidate, which may induce a long lasting immunity. Objectives: Production and partial characterization of seven monoclonal antibodies against Leishmania tropica promstigotes. Methods: Seven murine monoclonal antibodies of the IgG isotype we...

Purification of monoclonal antibodies by hydrophobic interaction chromatography under no-salt conditions

Hydrophobic interaction chromatography (HIC) is commonly used as a polishing step in monoclonal antibody purification processes. HIC offers an orthogonal selectivity to ion exchange chromatography and can be an effective step for aggregate clearance and host cell protein reduction. HIC, however, suffers from the limitation of use of high concentrations of kosmotropic salts to achieve the desire...

Hydrophobic Interaction Chromatography